Southern Blot At Biology Glossary
What is it? Transfer of electrophoretically separated fragments of DNA, after denaturation, from the gel to an absorbent sheet of material, such as nitrocellulose, to which the DNA binds. The sheet is immersed in a solution containing a labeled probe that will hybridize to fragment(s) of interest. The method was first devised by E. M. Southern to transfer DNA fragments from an agarose gel to a nitrocellulose paper for hybridization, but similar transfer methods are now also used for transfering RNA or protein to papers of a variety of types followed by hybridization (RNA) or labeled antibody treatment (protein) to identify specific molecules.Bio Definition Added By: Erin
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