RNA In Situ Hybridization At Biology Glossary

What is it? A technique that is used to identify the spatial pattern of expression of a particular transcript (usually an mRNA). In this technique, the probe is labeled, either radioactively or by chemically attaching a fluorochrome visualised by fluorescence or an enzyme that can convert a substrate to a visible dye. A tissue or organism is soaked in a solution of single-stranded probe under conditions that allow the probe to hybridize to complementary RNA sequences in the cells; unhybridized probe is then removed. Radioactive probe is detected by autoradiography. Fluorochrome is detected by fluorescence microscopy. Enzyme labeled probe is detected by soaking the tissue in the substrate; the dye develops in sites where the transcript of interest was expressed.

Bio Definition Added By: Jacob

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