Acrylamide At Biology Glossary

What is it? Used to make polyacrylamide gels for separation of macromolecules by electrophoresis. Polyacrylamide gels are produced by polymerization of acrylamide into linear chains and cross-linking the acrylamide chains with bis-acrylamide (N,N-methylene-bis-acrylamide). Polymerization is initiated by adding ammonium persulfate and the reaction is accelerated by TEMED (N,N,N,N-tetramethylethylenediamine) which catalyzes the formation of free radicals from ammonium persulfate. Acrylamide gels are generally poured between glass plates because oxygen inhibits the polymerization because oxygen radicals formed can interact with acrylamide and terminate chain elongation.

Bio Definition Added By: Kevin

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